Cloning of DNA Fragment involved Avirulence Gene of Xanthomonas campestris pv. campestris Strain Xcc1067 in Arabidopsis thaliana (L.) Heynh;
克隆包含以拟南芥为寄主的甘蓝黑腐病菌Xcc1067无毒基因的DNA片段
A pLAFRl:: Tn5gusA5 transposon has been constructed that fuse the Escherichia coli gusA reporter gene on cosmid pLAFRl, it was used to mutagenize wild type of Xanthomonas campestris pv.
构建携带报告基因的转座子Tn5gusA5于广谱性cos质粒pLAFR1上,利用Tn5gusA5诱变野生型甘蓝黑腐病黄单胞菌,筛选与致病性有关的因子(胞外多糖,胞外酶)突变体,通过Southern杂交和报告基因gus表达验证,突变体是由转座子诱变所致。
It has been shown that the extracellular protease I produced by Xanthomonas campestris pv.
甘蓝黑腐病黄单胞菌(XanthomonascampestrisPv。
A recombinant plasmid PGXN3000 containing a cluster genes of regulation of pathogenicity factor (rpf genes) of Xoo, was introduced into strain 8476, a mutant of rpfA gene of Xanthomonas campestris pv.
通过三亲本接合的方法,将获得的含水稻白叶枯病黄单胞菌rpf基因簇的重组质粒pGXN3000导入甘蓝黑腐病黄单胞菌rpfA基因突变体8476中。
This evidence indicates that O.
用甜菜黑色焦枯病毒(Beet Black Scorch Virus,BBSV) 直接接种甜菜根,BBSV轻度侵染甜菜;而将BBSV与甘蓝油壶菌( Olpidium brassicae) 游动孢子体外混合接种甜菜根,BBSV 则严重侵染。
Using AFLP silver staining method, we isolated five DNA markers from a pair of nearisogenic cauliflower lines, four of which are linked to the cabbage black rot ( Xanthomonas campestris pv.
这意味着该标记可能与甘蓝黑腐病抗性基因紧密连锁。
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