Concentration of total RNA recovered was evaluated by OD260/280 and agarose gel electrophoresis.
实验采用Trizol一次法或试剂盒提取猪精子的总RNA,然后通过RT-PCR扩增的方法,检测精子和睾丸组织中鱼精蛋白(PRM-1)的mRNA;使用不同数量的精子,检测在本实验条件下能扩增出PRM-1带所需要的最小精子数量;通过OD260/280的测算,判断不同提取方法所得RNA的纯度;通过琼脂糖电泳得到精子RNA与睾丸、卵巢组织RNA的电泳图。
Total RNA recovered was evaluated by agarose gel electrophoresis.
5×107精子可得到清晰的PRM-1条带;本试验首次得到猪精子总RNA的普通琼脂糖胶电泳图。
ELECTROPHORESIS PATTERNS OF BLOOD PLASMA PROTEIN POLYMORPHISMS AND THE ASSOCIATION WITH QUANTITATIVE OF DUCK;
鸭血浆蛋白(酶)多态性电泳图谱及其与数量性状的关系
Then,we gained some electrophoresis pattern of the prolamine which extracted from these three kinds of samples by SDS-PAGE(sodium dodecyl sulfate-polyacrylamide gel electrophoresis).
通过收集宁波口岸进口的香米、大米以及白糯米3种样品,用SDS—PAGE(十二烷基硫酸钠-聚丙烯酰胺凝胶电泳)鉴定方法,确定了上述样品醇溶蛋白的电泳图谱,并在此基础上构建了各样品醇溶蛋白在B、C两组区间的电泳图谱。
Studies on drawing of electrophoregrams aided by general computer with printer;
用普通电脑-打印机描绘电泳图谱的研究
PCR experimental system which is suitable for random amplified polymorphic DNA(RAPD) analysis of eggplant was developed by PCR(MJ/PTC-200) and electrophoregrams were analyzed, and two eggplant hybrids (Longzaqie No.
以龙杂茄2号、佳杂茄1号2个茄子杂种及其父母本为材料,使用MJ/PTC-200型PCR仪并对电泳图谱分析, 建立了适合茄子随机扩增多态性DNA分析的PCR扩增反应的体系,从40个随机引物中找到了2个可用于鉴定龙杂茄2号、3个可用于鉴定佳杂茄1号杂种纯度的引物。
The results revealed that the best resolving power was the electrophoregram obtained from the gel in the crosslinking index of 3.
以栽培大麦和中国近缘野生大麦为材料 ,用复合提取剂 (内含 2 5 % 2 -氯乙醇和 1 % 2 -巯基乙醇 )对其种子醇溶蛋白过夜提取后制备的样本液 ,在保持凝胶浓度 ( 1 8% )不变的基础上 ,通过改变交联度 ,催化剂用量 ,进样量以及电场强度和电泳时间所获得的不同电泳图谱 ,经比较分析可知 ,在酸性聚丙烯酰胺凝胶电泳体系中 ,交联度为 3 。
?The image features of corn seed protein electrophoregram was studied and a method, iterative computing thrade-off value in boundary detecting and regression analysis in image sharpening, of its lane boundary detection was proposed, which resulted in resolution of the problem of the lane boundary detection.
对玉米种子蛋白电泳图谱的特点进行了分析,在此基础上进一步提出电泳图谱图象中泳道边界的分析方法──边界提取时的阈值迭代技术及其相关的图象锐化回归技术。
Analysis of electrophoretic patterns of garlic somaclones;
大蒜体细胞无性系的电泳图谱分析
The Computer Analysis and Research of Gel Electrophoresis Image;
凝胶电泳图像计算机分析与实验研究
Results: There were obvious differences among the electrophoresis spectrums of the four types of seeds.
结果:4种种子的蛋白质电泳图谱具有明显差异。
The patterns of DNA of these viruses are similar to that of enteric adenovirus( EAd).
这些腺病毒DNA的电泳图型类似于肠道腺病毒。
Study on Computational Methods of Chromatogram and Two Dimensional Electrophoresis Image;
色谱及双向电泳图谱的计算解析方法研究
Computer-Based Identification on DNA Electrophoresis Map of Acipenser Sinensis;
中华鲟微卫星DNA电泳图谱的计算机自动识别
STUDY OF DIFFERENTIAL PROTEIN EXPRESSION IN HUMAN NORMAL LIVER CELLS EXPOSED TO RADIATION
辐射致人正常肝细胞蛋白质组电泳图谱的改变
The Preliminary Establishment of Two-dimensional Electrophoresis Map for Stomach Proteome of Coelomactra Antiquata
西施舌胃蛋白质组双向电泳图谱的初步构建
Establishment of 2-DE Map and Identification of Differential Protein of Bovine Whey from Healthy Cows and from Those with Clinical Mastitis by LC-MS/MS;
临床型乳房炎奶牛乳清蛋白质双向电泳图谱的建立及质谱分析
Differential Protein Profiles in 2-DE of Spinal Cord in Rat Fetus with Spina Bifida and Anorectal Malformation;
先天性脊柱裂和肛门直肠畸形脊髓蛋白质双向电泳图谱差异分析
Establishment of 2-DE Proteome Profile of Nasopharyngeal Secretion with Nasopharyngeal Carcinoma
人鼻咽癌鼻咽分泌物蛋白双向凝胶电泳图谱的建立
Preliminary analysis of cerebral proteins of mice with acute Toxoplasma gondii infection by 2D electrophoresis
急性弓形虫感染小鼠脑蛋白质二维电泳图谱初步分析
The Study on Construction and Application of TCM-PEF;
中药电泳指纹图谱的构建与应用研究
Investigation of Fingerprint Chromatography of Chinese Rose by HPCE;
中药月季毛细管电泳指纹图谱的研究
Capillary electrophoresis fingerprints of Compound Danshen Dropping Pill
复方丹参滴丸的毛细管电泳指纹图谱
Study on Characteristic Fingerprint of Radix Puerariae by Capillary Electrophoresis
中药葛根的毛细管电泳指纹图谱研究
A strip or band of electrophoretic medium showing the pattern of enzymes or isoenzymes after their separation by electrophoresis.
酶谱电泳媒质的谱带,电泳分裂后显出酶或同酶素的图案
Study on Fingerprints of Acanthopanax Senticosus (Rupr.et Maxim) Harms and Ephedrae Herba by Capillary Electrophoresis;
中药刺五加和麻黄的毛细管电泳指纹图谱研究
Study on the Functional Fingerprint of Traditional Chinese Medicine by Capillary Electrophoresis;
功能化的毛细管电泳中药指纹图谱研究
CopyRight © 2020-2024 优校网[www.youxiaow.com]版权所有 All Rights Reserved. ICP备案号:浙ICP备2024058711号