The amino acid sequence of glyceraldehyde-3-phosphate dehydrogenase(GAPDH)(AAN09371) from Drosophila melanogaster was used to tblastn searching EST of Bombyx mori.
从家蚕EST数据中检索到果蝇(Drosophila melanogaster)甘油醛-3-磷酸脱氢酶(GAPDH)(AAN09371)氨基酸同源序列,用seqMAN延伸,电子克隆家蚕gapdh的cds,用PCR克隆家蚕gapdh基因序列。
These enzymes measured were glyceraldehydes-phosphate dehydrogenase (GAPDH), glycerol-3-phosphate dehydrogenase (GDH), lactate dehydrogenase (LDH), and 3-hydroxyacyl-CoA dehydrogenase (HOAD).
对3日龄粘虫雌蛾吊飞过程中4种相关酶3-羟酰辅酶A脱氢酶(HOAD)、3-磷酸甘油醛脱氢酶(GAPDH)、3-磷酸甘油脱氢酶(GDH)和乳酸脱氢酶(LDH)的研究结果表明,在室内条件下,粘虫在吊飞过程中其能量代谢有以下特点:在吊飞的初始5min,所有与糖代谢和脂肪代谢相关的酶活性都快速升高,这段时期脂肪代谢的酶活性也完全被活化,HOAD活性明显增强;但在随后的5~60min持续吊飞期间与能量代谢有关的酶活性都有所下降,表明此时飞行活性趋于平稳。
MTT assay was used to measure cell viability, flow cytometry and TUNEL assay were used to test cell apoptosis, and Western blot was used to detect the expression of GAPDH.
方法采用100nmol/L鱼藤酮作用SH-SY5Y细胞24h,建立SH-SY5Y细胞凋亡模型;再用100、200、300μmol/L不同浓度的利福平进行干预,采用MTT比色法检测细胞活性,采用流式细胞术(FCM)及原位缺口末端标记染色法(TUNEL)检测细胞凋亡,Western blot法检测前凋亡蛋白3-磷酸甘油醛脱氢酶(GAPDH)的表达水平。