A turbidimetry assay for fast determination of colistin sulfate was developed.
以大肠杆菌为试验菌,抗生素检定培养基Ⅲ号为试验用培养基,研究微生物比浊法测定硫酸黏菌素及其制剂的含量。
Turbidimetry assay for fast determination of apramycin sulfate,tylosin,colistin sulfate were developed.
将硫酸安普霉素、泰乐菌素、硫酸黏菌素稀释成不同的浓度加入菌悬液中,37℃培养3~4h,确立浓度的对数值与相对应的吸光度呈直线相关的线性范围,并对所建立的浊度法进行准确度、精密度、浊度法与管碟法比较及专属性等验证。
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